erk1 2 sirna (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
erk1 2 sirna
Erk1 2 Sirna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erk1+sirna/SignalSilence+p44%2F42+MAPK+(Erk1%2F2)+siRNA/pm41319747-57-32-38
Average 94 stars, based on 109 article reviews
Erk1 2 Sirna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erk1+sirna/SignalSilence+p44%2F42+MAPK+(Erk1%2F2)+siRNA/pm41319747-57-32-38
Average 94 stars, based on 109 article reviews
erk1 2 sirna - by Bioz Stars,
2026-09
94/100 stars
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Construct:Article Title: Oncogenic BRAF V600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase Article Snippet: After transduction with BRAF V600E , BRAF WT , and vector lentivirus, respectively, for 24 hours, cells were cultured for 24 hours in DMEM/F12 without fetal bovine serum (FBS), and then medium and cells were collected for subsequent experiments. .. Constructs used were: pMD2.G (Addgene, Cat# 12259, RRID: Addgene_12259), psPAX2 (Addgene, Cat# 12260, RRID: Addgene_12260), pHAGE- BRAF V600E plasmid (Addgene, Cat# 116204, RRID: Addgene_116204), pHAGE- BRAF WT plasmid (Addgene, Cat# 116719, RRID: Addgene_116719), pBabe-Puro- BRAF V600E plasmid (Addgene, Cat# 15269, RRID: Addgene_15269), gag/pol-Retroviral plasmid (Addgene, Cat# 14887, RRID: Addgene_14887), Control siRNA (Cell Signaling Technology, Boston, MA, USA, Cat# 6568S), SAPK/JNK siRNA (Cell Signaling Technology, Cat# 6232S), Article Title: Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK Article Snippet: .. Methods Constructs and viral vectors Constructs used were: pMD2.G (Addgene, Cat# 12259, USA), psPAX2 (Addgene, Cat# 12260, USA), pHAGE-BRAFV600E plasmid (Addgene, Cat# 116204, USA), pHAGE-BRAFWT plasmid (Addgene, Cat# 116719, USA), pBabe-Puro-BRAFV600E plasmid (Addgene, Cat# 15269, USA) , gag/pol-Retroviral plasmid (Addgene, Cat# 14887, USA), Control siRNA (Cell Signaling Technology, Cat# 6568S, USA), SAPK/JNK siRNA (Cell Signaling Technology, Cat# 6232S, USA), Plasmid Preparation:Article Title: Oncogenic BRAF V600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase Article Snippet: After transduction with BRAF V600E , BRAF WT , and vector lentivirus, respectively, for 24 hours, cells were cultured for 24 hours in DMEM/F12 without fetal bovine serum (FBS), and then medium and cells were collected for subsequent experiments. .. Constructs used were: pMD2.G (Addgene, Cat# 12259, RRID: Addgene_12259), psPAX2 (Addgene, Cat# 12260, RRID: Addgene_12260), pHAGE- BRAF V600E plasmid (Addgene, Cat# 116204, RRID: Addgene_116204), pHAGE- BRAF WT plasmid (Addgene, Cat# 116719, RRID: Addgene_116719), pBabe-Puro- BRAF V600E plasmid (Addgene, Cat# 15269, RRID: Addgene_15269), gag/pol-Retroviral plasmid (Addgene, Cat# 14887, RRID: Addgene_14887), Control siRNA (Cell Signaling Technology, Boston, MA, USA, Cat# 6568S), SAPK/JNK siRNA (Cell Signaling Technology, Cat# 6232S), Article Title: Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK Article Snippet: .. Methods Constructs and viral vectors Constructs used were: pMD2.G (Addgene, Cat# 12259, USA), psPAX2 (Addgene, Cat# 12260, USA), pHAGE-BRAFV600E plasmid (Addgene, Cat# 116204, USA), pHAGE-BRAFWT plasmid (Addgene, Cat# 116719, USA), pBabe-Puro-BRAFV600E plasmid (Addgene, Cat# 15269, USA) , gag/pol-Retroviral plasmid (Addgene, Cat# 14887, USA), Control siRNA (Cell Signaling Technology, Cat# 6568S, USA), SAPK/JNK siRNA (Cell Signaling Technology, Cat# 6232S, USA), Control:Article Title: Oncogenic BRAF V600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase Article Snippet: After transduction with BRAF V600E , BRAF WT , and vector lentivirus, respectively, for 24 hours, cells were cultured for 24 hours in DMEM/F12 without fetal bovine serum (FBS), and then medium and cells were collected for subsequent experiments. .. Constructs used were: pMD2.G (Addgene, Cat# 12259, RRID: Addgene_12259), psPAX2 (Addgene, Cat# 12260, RRID: Addgene_12260), pHAGE- BRAF V600E plasmid (Addgene, Cat# 116204, RRID: Addgene_116204), pHAGE- BRAF WT plasmid (Addgene, Cat# 116719, RRID: Addgene_116719), pBabe-Puro- BRAF V600E plasmid (Addgene, Cat# 15269, RRID: Addgene_15269), gag/pol-Retroviral plasmid (Addgene, Cat# 14887, RRID: Addgene_14887), Control siRNA (Cell Signaling Technology, Boston, MA, USA, Cat# 6568S), SAPK/JNK siRNA (Cell Signaling Technology, Cat# 6232S), Article Title: Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK Article Snippet: .. Methods Constructs and viral vectors Constructs used were: pMD2.G (Addgene, Cat# 12259, USA), psPAX2 (Addgene, Cat# 12260, USA), pHAGE-BRAFV600E plasmid (Addgene, Cat# 116204, USA), pHAGE-BRAFWT plasmid (Addgene, Cat# 116719, USA), pBabe-Puro-BRAFV600E plasmid (Addgene, Cat# 15269, USA) , gag/pol-Retroviral plasmid (Addgene, Cat# 14887, USA), Control siRNA (Cell Signaling Technology, Cat# 6568S, USA), SAPK/JNK siRNA (Cell Signaling Technology, Cat# 6232S, USA), Article Title: The clinically approved MEK inhibitor Trametinib efficiently blocks influenza A virus propagation and cytokine expression. Article Snippet: The siRNA transfections of A549 cells were conducted using Lipofectamine® 2000 transfection reagent (Invitrogen) according to the manufacturer ́s protocol using a final siRNA concentration of 20 nM. .. Transfection:Article Title: Oncogenic BRAF V600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase Article Snippet: .. Primary neurons and microglial cells were seeded in 6-well plates and transfected with Article Title: Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK Article Snippet: .. All experiments were performed in accordance with a protocol approved by Institutional Animal Care and Use Committee at Massachusetts General Hospital. siRNA transfection Primary neurons and microglial cells were seeded in 6-well plates and transfected with |